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DOT Drug Testing Requirements

The Department of Transportation outlines strict drug testing requirements for anyone executing a “safety sensitive” position within the transportation industry.

Some of the requirements include strict regulations regarding:

  • Urine sample collection (specimen collectors, collection sites, detailed procedures)
  • Breath alcohol collection procedures
  • Laboratory procedures (forms, equipment, supplies to use for urine collection)
  • Reporting (medical review officers and verification process)
  • Record keeping of final results (confidentiality and release of information) 

Be sure to read all regulations outlined in the DOT’s rule 49 CFR Part 40.

Testing Procedures

Transmetron - A Qualified DOT-drug Testing Facility

Transmetron is a full-service Third Party Provider (TPP) for DOT urine collections for drug testing. We can either collect the specimen(s) on-site in our facility, or our mobile team can perform the collection(s) on-site.

We follow procedures outlined by the Department of Health and Human Services (DHHS). Only labs qualified by SAMHSA may be used for DOT drug testing requirements. We ensure that each step we take safeguards the accuracy of the testing being performed and protects both drivers and passengers alike.

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What Are the Testing Procedures?

The official drug and DOT urine testing procedures are based on those established by the Department of Health and Human Services. These procedures include: urine sample collection, laboratory procedures, and reporting and record keeping of final results. Only laboratories qualified by Health and Human Services under the National Laboratory Certification Program (NLCP) may be used. These procedures exist to safeguard accuracy and to protect the privacy of drivers.

The Omnibus Transportation Employee Testing Act of 1991 requires drug and alcohol testing of safety-sensitive transportation employees in aviation, trucking, railroads, mass transit, pipelines, and other transportation industries.

Transmetron offers fully DOT-compliant drug testing services as established by 49 CFR Part 40.

We also offer the following Federal DOT-compliant services in addition to our Drug Testing Services:

  • On-site testing
  • Consortiums
  • Policy creation
  • Audit Preparation
  • Alcohol testing
  • Random name generation
  • Training
  • Post Accident testing
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Coast Guard (USCG) DOT Drug Testing Requirements

The Coast Guard exists to enforce maritime law and to ensure maritime safety, security, and stewardship.

Because the Coast Guard is both a part of a federal regulatory agency and a military drug test service, its mission is unique. To maintain a safe environment for all Coast Guard service members, personnel, and the public, the Coast Guard must follow strict regulations.

The United States Coast Guard must abide by the regulated-employer DOT policies. Transmetron employees are certified to perform these DOT drug and alcohol testing procedures and requirements.

DOT Alcohol Testing Requirements

In addition to strict drug and DOT urine testing requirements, the Department of Transportation also requires employers to perform pre-employment, random, post-accident, or reasonable suspicion alcohol screening on any individual carrying out a “safety-sensitive” role.

Transmetron can assist you in meeting DOT alcohol testing standards, ensuring full compliance with these regulations.

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Ensuring Your DOT Alcohol Testing Compliance

The DOT’s rule 49 CRF Part 40 explains requirements for DOT alcohol testing including the following:

  • Only certain personnel – Screening Test Technician (STTs & Breath Alcohol Technicians (BATs) may conduct DOT alcohol screening
  • Alcohol screening must be documented with forms specially furnished by the DOT
  • Only certain devices are approved for use in DOT alcohol screening & confirmation testing
  • Required steps for a DOT alcohol screening and DOT alcohol confirmation test
  • Procedures for handling results


Here at Transmetron we understand the importance of DOT compliance and we know the regulations for the alcohol screening. We are happy to help your organization achieve complete compliance with DOT procedures.

Certified Professionals

Every technician at Transmetron is certified to perform DOT-regulated tests, which means that any organization or person needing a DOT alcohol test may use our services.

Federal Motor Carrier Safety Administration (FMCSA) Drug Testing Requirements

The Federal Motor Carrier Safety Administration is dedicated to improving the safety of commercial motor vehicles (CMV) and saving lives. If your organization is regulated by the FMCSA, then you are required to follow their regulations for federal job drug testing. Transmetron can help you navigate these complex laws and implement an FMCSA-compliant drug testing program.

Federal Aviation Administration (FAA) Drug Testing Requirements

We now offer “Flight School” drug tests!
The Federal Aviation Administration (FAA), as part of the Department of Transportation, aims to provide the safest, most efficient aerospace system in the world. In order to create a safe environment, the FAA must regulate the certification of aircraft, airlines, and all personnel.

The FAA works to keep consumers, goods, aircraft, pilots, and mechanics safe. Maintaining a safe airspace requires productive workers. The FAA ensures that all airspace personnel are abiding by the DOT guidelines. Among other policies is the necessity of drug and alcohol testing.

All technicians that work at Transmetron are approved and certified to conduct DOT drug and alcohol testing, according to FAA standards. We perform tests on-site and have a mobile team to perform collections at your location.

Federal Railroad Administration (FRA) Drug Testing Requirements

The Federal Railroad Administration (FRA) exists to provide safe, reliable, and efficient rail transportation solutions for people and goods. If you are regulated under the FRA, it’s your responsibility to be aware of the DOT drug testing requirements so you can remain compliant.

Transmetron can assist you with your FRA drug testing needs. Call us to learn more at 801-596-2709

Overview of FMCSA Drug & Alcohol Regulations

To ensure that drivers, passengers, and pedestrians are safe, the FMCSA maintains strict drug and alcohol testing requirements. There are regulations for the driver, vehicle, and company. All regulations are in place to reduce risk to lives, property, and the environment.

Transmetron can gladly help companies and drivers follow the FMCSA guidelines by offering drug and alcohol testing services that are compliant with DOT policies.

Federal Transit Administration (FTA) Drug Testing Requirements

The Federal Transit Administration (FTA), as a part of the Department of Transportation (DOT), provides financial and technical assistance to local public transit systems. The main goals of this administration are to help new transit systems and improve and maintain the current transit systems across the country.

Compliance With FRA Drug Testing Requirements

The FRA strives to display integrity and excellence in all decisions, including regulations pertaining to FRA drug testing. Because the administration is focused on preserving a trusting relationship with the public, the creation of a safe and efficient railroad system is vital. To maintain a safe environment for all employees and the public, the FRA must abide by the DOT regulations. Keeping employees sober and safe is a top priority, and FRA-regulated federal job drug testing is one way to ensure this.

Know the Rules for Pipeline & Hazmat Drug Testing

The Department of Transportation maintains strict DOT drug and alcohol testing requirements for anyone involved in the rehabilitation, repair, or replacement of pipelines. These include rules for how consortium testing and hazmat certification can be conducted.

Performing these safety-sensitive tasks requires employees to undergo DOT workplace drug and alcohol testing and requirements. Strict guidelines ensure the safe and secure movement of hazardous materials. These requirements, which include regular drug and alcohol testing, are in place to reduce risks of threats to life, property, and the environment.

Pipeline & Hazardous Materials Safety Administration (PHMSA) Drug Testing Requirements

If your organization falls under the Pipeline & Hazardous Materials Safety Administration, or PHMSA, then you must ensure that you are aware of all federal job drug testing regulations.

Here at Transmetron, we are equipped to perform all of your PHMSA-related drug and alcohol tests. Whether you are a smaller organization interested in participating in a testing consortium or an employer with individuals pursuing hazmat certification, you will need to conduct PHMSA drug testing